ANCIENT DNA

[Mic Chaudoir]

In article <cmeghen-171094154018 at gen023.gen.tcd.ie>, cmeghen at mail.tcd.ie
(Ciaran Meghen) wrote:

> QUESTION? What do YOU think of using autoclaving as a technique to wholly
> or partially remove contaminating nucleic acids from solutions. Does it
> work? I do it anyway just because I'm paranoid.
> 
> ************************************************************************
> Chris Troy,           E-mail: catroy at mail.tcd.ie
> Genetics Department,     Phone:  (353)-1-7021265
> Trinity College,         Fax:    (353)-1-6798558
> Dublin 2.
> Ireland.
> *************************************************************************

As others may have said previously, Autoclaving will NOT remove
contaminating DnA from solutions.  This is one area where properly
cleaning glassware with water will help, a lot.  Since DNA is water
soluble, properly cleaned and sterilized glassware should be OK.  However,
dH2O often has bacteria in it, and thus DNA.  If you are paranoid, do a
few rinses with ddH2O (like mili-Q or something).

-- 
mic
mic at nwu.edu

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