Adding membrane signal to cytoplasmic protein

[George W Chacko]

In article <1994Oct25.210606.8762 at mbcf>,  <mhughes at mbcf.stjude.org> wrote:

> You might want to try adding a ras-like CAAX sequence to the
> c-terminus of you protein.  I don't know if this has been done
> successfully before or not, but I'm almost positive that this sequence
> alone comprizes the entire myristilation signal for ras and many
> other,proteins, and may work for armadillo.

Correct me if I'm wrong but is a CAAX box really a myristilation
signal? I was underthe impression that myristilation is directed by a
glycine in position 2 (ref: Marilyn Resh's recent minireview in Cell).


George Chacko