Adding membrane signal to cytoplasmic protein

[mhughes at mbcf.stjude.org]

In article <01HIIC3VA6HU0008KV at UNCVX1.OIT.UNC.EDU>, ORSULIC at UNCVX1.OIT.UNC.EDU writes:
> I am working on structure/function analysis of Drosophila Armadillo protein.
> The protein is about 105 kb, does not have a signal or transmembrane sequence,
> and is mostly cytoplasmic. 
> 
> I would like to generate the form of the protein that would be tethered to the
> membrane. Any suggestions, protocols or references of successful addition of a
> myristilation or membrane signal to other proteins would be appreciated. 
> 
> 
> Sandra Orsulic
> University of North Carolina
> 
> orsulic at uncvx1.oit.unc.edu

Sandra
	You might want to try adding a ras-like CAAX sequence to the c-terminus
of you protein.  I don't know if this has been done successfully before or not, 
but I'm almost positive that this sequence alone comprizes the entire
myristilation signal for ras and many other proteins, and may work for
armadillo.  
	Good luck,

	Mark Hughes
	St. Jude Children's Research Hospital
	MHUGHES at mbcf.stjude.org