Total cellular protein vs cell number

Yasuhito Abe yasuhito at ME1SERV.M.EHIME-U.AC.JP
Mon Mar 6 00:49:31 EST 1995


At  8:30 PM 95.3.5 -0600, Stephen Gately wrote:
>I am trying to quantitate a cell-associated protein, and want to
>standardize it.  Does anyone have any suggestions on whether I should
>standardize to cell number or to total cellular protein?
>
>Thanks

Dear Sir;
        If your cells can be applied to the flow-cytometric analysis,
 you can use FACS machine to semi-standardize surface membrane expressed 
or associated protein using antibodies to them.  You can refer to the 
"Current Protocols in Immunology" or other text books.
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