Mobility Shift and Glutamic Acid

[Stefan Einar Stefansson]

Hi

I am currently doing mobility shift on purified transcriptional
factor. By substituting the potassium chloride for potassium
glutamic acid, I can get much stronger shift. Unfortunately,
the free probe, barely separates from the one being shifted,
when run on the gel.

Has anyone encountered this problem, and/or knows how to get
a better separation, of the free probe from the shifted complex?

Hope to hear from as many as possible,

Stefan
stefan at sickkids.on.ca