Freezing Bacteria-Liquid N2 OK?

[Kimberly Baldwin]

Hello All,

I was wondering about the feasability of storing frozen bacterial stocks
under liquid nitrogen vs. storing at -80oC.  Are the cells viable?  Should
they first be frozen slowly prior to storing under liquid nitrogen?  Or is
snap freezing them okay?  Should they be frozen as glycerol stocks-or is
DMSO or DMF okay to use instead?  I have always in the past stored them at
-80oC (in glycerol or  with DMSO). 

I appreciate any and all responses-thanks in advance.

Kimberly Baldwin

kbal at med.pitt.edu

-- 
Kimberly Baldwin
kb44+ at andrew.cmu.edu