RNA from Spleen - Help!

Karl Fischer kfischer at gpu.srv.ualberta.ca
Tue Mar 21 14:04:42 EST 1995


In article <3knc2r$ee0 at netnews.upenn.edu>, aaron at ccat.sas.upenn.edu (Aaron
Pawlyk) wrote:

>Spleens are removed from freezer and pulverized in liquid nitrogen in a
>mortar and pestle.

I'm assuming the freezer is -70 to -80C...Try freezing the spleen in
liquid nitrogen after removal and storing it in a cryochamber until
needed. Pancreatic tissue needs a similar anal-retentive approach because
of all those dang RNAses;-)

Karl

-- 
Karl Fischer
kfischer at gpu.srv.ualberta.ca
tyr-2 at bones.biochem.ualberta.ca



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