PCR subcloning

[Jillian Johnston]

I was about to ask the same question myself today!  I would like
to subclone my Taq-generated PCR product into a vector that will not be its
final resting place (its a long story).  I was advised to use a
T-vector which I dutifully made (very easy and cheap) and when
faced with trying to determin vector:insert ratio aI thought it
would be important to know just what % of the insert actually has
a single 'A' on each end.  To my dismay I read that one could
expect something like 9% to be what I wanted for cloning.  Is
this really the case and if so why go to the trouble of using
T-tailed vectors if most of the PCR product is blunt-ended?  I've
gone ahead with it anyway but would like some advice for the next
time I do this which will probably be in a couple of days.
Thanks

--
Jillian Johnston
Dept. of Biological Sciences       Guider
Univ. of Calgary         	   30 Pathfinders, Tanisi District