Prepping Plasmids for Automated Sequencing?

[John Watson]

J. Pat Martinez wrote:
> 
> Any suggestions on miniprep columns for purifying plasmid DNA to be used
> for automated sequencing?  I was considering buying the mini prep spin
> columns from Qiagen or the perfect prep columns from 5 prime 3 prime.

We routinely use Qiaprep 8 vacuum manifold columns ass well as Promega Wizard 
Kits and find that both give reliable automated sequence.

AJW

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John Watson
Bristol-Myers Squibb Co.
watson_j at bms.com
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"If you're not part of the solution, you're part of the precipitate."
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