Mammalian cell transfection

[Vincent Solomon]

Mike Garbutt (mgarbutt at gpu.srv.ualberta.ca) wrote:
: LABO DE BIOCHIMIE wrote:
: > 
: > Hi folks,
: > I am planning to transfect mammalian cells by using the classical
: > calcium phosphate procedure with the recombinant expression vector
: > pCI-neo (Promega), which confers by its own G-418 resistance to
: > transfected cells.

: I would suggest using "DOSPER" and do transients if you can get away with 
: it; you can get almost 100% transfection efficiency, as long as you don't 
: kill all your cells :-(
: Mike
: -- 
: Mike Garbutt,
: Dept. of Anatomy and Cell Biology,
: University of Alberta.
: e-mail:   mgarbutt at gpu.srv.ualberta.ca
: homepage: http://www.ualberta.ca/~mgarbutt/

: Industry without education is blind; education without industry is lame.

What is DOSPER?  --