Mammalian cell transfection
vsolomon at fas.harvard.edu
Fri Jan 10 18:52:39 EST 1997
Mike Garbutt (mgarbutt at gpu.srv.ualberta.ca) wrote:
: LABO DE BIOCHIMIE wrote:
: > Hi folks,
: > I am planning to transfect mammalian cells by using the classical
: > calcium phosphate procedure with the recombinant expression vector
: > pCI-neo (Promega), which confers by its own G-418 resistance to
: > transfected cells.
: I would suggest using "DOSPER" and do transients if you can get away with
: it; you can get almost 100% transfection efficiency, as long as you don't
: kill all your cells :-(
: Mike Garbutt,
: Dept. of Anatomy and Cell Biology,
: University of Alberta.
: e-mail: mgarbutt at gpu.srv.ualberta.ca
: homepage: http://www.ualberta.ca/~mgarbutt/
: Industry without education is blind; education without industry is lame.
What is DOSPER? --
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