Long PCR Primers

[Andrew Doherty]

Sandra Pena De Ortiz wrote:
> 
> I am thinking on using a 40 bp long pcr primer.  I need to add a
> consensus Kozak sequence for expression and want to add a bamhi site to
> ease cloning.  Does anyone know if a primer this long will work?  The
> longest I have tried is 26 and had no problems.  Also, how many bp minimum
> should
> I add 5' to the bamhi site to ensure binding and cleavage by the enzyme?
> 
> Thanks in advance,
> Sandra Pena de Ortiz, Ph.D.
> Dept. of Biology, UPR
HI Sandra,

I've used PCR primers of that sort of length and they're fine - I always
have at least 15 bp of complemetary sequence and the rest can be
anything you want! BamHI only needs a  couple of bases 5' for cutting.

Hope it helps
Any D 
-- 
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Dr Andrew Doherty		email -  a.doherty at bris.ac.uk
Dept. Anatomy			Tel (0117)9287421
School of Medical Sciences	Fax (0117)9287402
University of Bristol
University Walk
Bristol UK
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