btf at t10.lanl.gov
Tue Jan 14 11:19:54 EST 1997
>... I'm currently trying to perform a
> phylogenetic analysis based on rbcL (for the large
> subunit of Rubisco) gene
> sequences. I have some sequences, but need some
> more to give the analysis more
Keep in mind that the more sequences you
have, the more computationally intensive the analysis
> The problem I have is obtaining rbcL sequences
> of green algae.
> ... In fact, sequences
> wich I know exist and have personally retrieved from
> genbank do not appear in the search results.
I have found that the best way to retrieve
a great many sequences from GenBank is to do a BLAST
search with my gene of interest. Not only do I get
the accession numbers for many homologs and related
genes, but they are also ordered by percent similarity
(at least in an ungapped alignment).
For information, send a message to
blast at ncbi.nlm.nih.gov with "help" as the
body of the e-mail message. There are also other
similar search engines available, but BLAST is a
good place to start.
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