XL1-RED mutator strain

[Ji-quan Liu]

At  0:20 PM 97.1.15 -0800, ebylina wrote:
>Does anyone have experience using the XL1-RED mutator strain from
>Stratagene?   I want to introduce random mutations in a ~800 bp gene.
>What problems and pitfalls have been experienced?  Does anyone know of
>references in which this strain was used to mutagenize a protein?


$B!d!d(BSee  In vitro mutagenesis protocols, methods in Molecular Biology, vol 5
7, Page 375, Contributed by Alan Greener et al.

I am also going to try the method, any experience please let me know, too.

Ji-Quan liu