Richard P Grant
rgrant at worf.molbiol.ox.ac.uk.remove_this_bit
Thu Jan 16 04:53:03 EST 1997
I'm designing oligos for mutagenesis. The mutagenesis will be done by the
polymerase method (eg Stratagene 'QuikChange'). It is, apparently,
advisable to have C or G at each end of the oligo.
The end of one of my - putative - oligos looks like ...CCATCACCC. I know
with sequencing that G/C clamps at the end are a Bad Idea. What worries
me is that 3Cs at the end might cause mis-priming. Would ...CCATCAC
work better? The latter case has 9 parent bases after the last mutant
site, which is the bare minimum for effective hybridization. Does anyone
have any comments?
Richard P. Grant MA DPhil University of Oxford
Nuffield Department Obstetrics and Gynaecology FFPGP
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