transient transfection:effect of plasmid dna?
hohoff at uni-muenster.de
Fri Jan 17 09:46:07 EST 1997
Did someone ever notice an influence of plasmid dna quality (here: ratio
covalently closed circular versus open circular dna) on transient transfection
of mammalian cells?
I noticed that two plasmid purification kits (both anion exchange types) had
markedly different yields of ccc plasmid dna vs oc.
Thank you very much in advance
Department of Biochemistry
University of Muenster
E-Mail: hohoff at uni-muenster.de
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