WANTED: lexA-lacZ reporter

Scott Vande Pol sbv at pop.cwru.edu
Sun Jan 19 12:03:56 EST 1997


In article <stebbin1.1203576394B at nntp.msu.edu>, stebbin1 at student.msu.edu
(John Stebbins) wrote:

> I am looking for a yeast reported plasmid that has the lacZ gene with
> upstream lexZ binding sites on a plasmid that is His3 marked.  If anyone has
> one or knows of one please contract me.  
> 
> Thanks!  John Stebbins
> --------------------------------------------------------------------------------
> 
>                 John Stebbins
>                 stebbin1 at pilot.msu.edu
>                 Michigan State University
>                 Dept. of Biochemistry
>                 (517) 432-3668
> --------------------------------------------------------------------------------


We made such a reporter that is the same as pSH18-34 but with HIS3
selection.  We also made a variety of lexA bait plasmids with URA3
selection so that they can be selected against with FOA.  
-- 
Scott Vande Pol
Case Western Reserve University
Department of Pathology
Cleveland Oh. 44106
sbv at pop.cwru.edu



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