WANTED: lexA-lacZ reporter
Scott Vande Pol
sbv at pop.cwru.edu
Sun Jan 19 12:03:56 EST 1997
In article <stebbin1.1203576394B at nntp.msu.edu>, stebbin1 at student.msu.edu
(John Stebbins) wrote:
> I am looking for a yeast reported plasmid that has the lacZ gene with
> upstream lexZ binding sites on a plasmid that is His3 marked. If anyone has
> one or knows of one please contract me.
>
> Thanks! John Stebbins
> --------------------------------------------------------------------------------
>
> John Stebbins
> stebbin1 at pilot.msu.edu
> Michigan State University
> Dept. of Biochemistry
> (517) 432-3668
> --------------------------------------------------------------------------------
We made such a reporter that is the same as pSH18-34 but with HIS3
selection. We also made a variety of lexA bait plasmids with URA3
selection so that they can be selected against with FOA.
--
Scott Vande Pol
Case Western Reserve University
Department of Pathology
Cleveland Oh. 44106
sbv at pop.cwru.edu
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