GC-Melt

[David Walker]

The January 1997 issue of Clontech's "CLONTECHniques" describes a new 
PCR kit called "Advantage-GC" which contains a new reagent which they 
call GC-Melt.  This reagent allows one to obtain PCR products from 
high GC templates. It destabilizes DNA and also isostabilizes DNA 
(makes AT and GC base pairs equally stable). They say it is not DMSO. 
Does anyone have any ideas as to what this stuff is?  I would buy some 
for a reasonable price, but it seems to only be offered as part of a 
$400 kit.

David Walker
David_Walker.GICD at quickmail.ucsf.edu