Dissolving plasmid DNA afterprecipitation

[Steven Toth]

Keven,

     What is your template isolation method.  If it is Quiagen or 
alike with a solid support column, I have seen where some of this 
support can come through into samples.  These then pellet down with 
ppt DNA and will not resuspend when dissolving your plasmid.  If you 
are using these methods of template prep then try a control of just 
H2O over the column and see if you get solid pellet/ppt.  Hope this 
helps, good luck.

Cheers 

Stephen Toth
Genome Therapeutics Corp.
100 Beaver St.
Waltham, MA  02154
tel:  617-893-5007 X2551
      617-398-2551 (Direct Dial)
fax:  617-893-9535
toth at cric.com