Phenol Protocol

[Lou Cantolupo]

Does anyone here have a good, reliable protocol for making buffered
phenol?  I currently use B-M phenol with 8-HQ added, buffered twice with
untitrated Tris base, then twice with Tric-Cl 8.0.  This works well, but
takes a good half day to do due to the separations when making 500mL at
a time.  Thanx in advance....

Lou Cantolupo
Dept. Molecular Virology
GenVec, Inc.
CANTOLUPO at GENVEC.COM