Alexander N. Kukushkin
kan at mmcd.cyt.ras.spb.ru
Wed Jan 29 13:06:55 EST 1997
> Does anyone here have a good, reliable protocol for making buffered
> phenol? I currently use B-M phenol with 8-HQ added, buffered twice with
> untitrated Tris base, then twice with Tric-Cl 8.0. This works well, but
> takes a good half day to do due to the separations when making 500mL at
> a time. Thanx in advance....
> Lou Cantolupo
> Dept. Molecular Virology
> GenVec, Inc.
> CANTOLUPO at GENVEC.COM
During many years we use successfully the following recipe of self-made
buffered phenol to isolate DNA:
1. Mix up 300 ml of distilled melted phenol and 150 ml of TE, pH 8.0.
2. Add 6.75 ml of 5M KOH and 8-hydroxyquinoline to 0.1%.
This mixture is quickly prepared and has pH 8.0-8.2.
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