oligo hybridization (degenerate oligo)
marc van de craen
marcvdc at LMB1.RUG.AC.BE
Mon Jun 2 12:33:53 EST 1997
We want to hybridize two thousand of SELECTED cDNAs with 2 degenerate
oligo's which only differ in one codon. One oligo (wtOLIGO) will
entirely code for the cDNAs we are looking for. The other oligo
(mutOLIGO) will contain specific mutations (at the level of the
different codon) which will certainly not be present in the cDNAs we are
looking for. By adjusting the washing conditions we want to select the
cDNAs which specifically hybridize with the wtOLIGO and not with the
mutOLIGO. We want to use the mutOLIGO to be sure that the hybridization
conditions with wtOLIGO are optimal for specific cDNAs.
1) Is it possible to do such experiments ?
2) Can someone advice us how to design the oligo's and how to do the
experiment (especially the washing conditions are of concern for us)
3) Is there literature describing analogous experiments ?
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