5' end labeling

[A. Kumar]

I am having trouble with 5' end labeling of a 28mer oligo. I am using a
molar ratio of 32P to substrate DNA of 3:1, and the specific activity of
the 32P-ATP is 6000 Ci/mmol. I was getting good labeling about a year ago,
and all of a sudden it stopeed working. If anyone has any suggestions or
ideas, I would really appreciate it.
thanks

Ashish Kumar
 
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