how many nucleotides are required for restriction enzyme digestion of biotinylated DNA?

Hahn Yoonsoo yshan at bioneer.kaist.ac.kr
Sun Jun 8 08:36:17 EST 1997


 I have to construct a linker which is biotinylated and will be cut by
restriction enzyme later. How many nucleotides are required between biotin
and the restriction site? Does biotin inhibit the enzyme if it is very close
to the restriction site?

  biotin-NNNNNNNGAATTCTTTTTTTTTTTTTTTT
         ^^^^^^^
         How many N's are needed?

Please email me the answer..

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