Is ligation reaction volume only 10 ul?

Nick Jacobsen jacobsen at cf.ac.uk
Tue Jun 17 04:24:17 EST 1997


Hi,
  You must keep the level of free water in your ligations low to ensure 
efficiency. However I have done ligations in 50microlitres and they 
worked fine. For situations when you need excellent efficiency (library 
prep) ligations are performed in 5microlitres

hope this helps

Nick




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