PCR reamplification problem

[Steven Sullivan]

One possible tweak to Dr. Sylvester's method is to run your original PCR
in a low-melt gel lacking EDTA in the gel or buffer (a 'TA' gel), then
melting and resuspeding the plug in a small volume of water.  This
eliminates the possibility of EDTA interfering with the Mg++
concentration. This is a tweak someone clued me into on this newsgroup
a few weeks back.