Dr. Duncan Clark
duncan at genesys.demon.co.uk
Thu Jun 26 03:32:32 EST 1997
In article <5oom1a$c5u at info.abdn.ac.uk>, "j.r.stephen"
<mbi071 at sysc.abdn.ac.uk> writes
>I have seen an advertisment at some point for a dye you add to PCR mixes to see
>a +/- reaction, ie. it only fluoresces if you get a product. Does anyone know
>what I'm talking about, or am I mixing-dreams and reality again?
SYBR-Green from Molecular probes. Even Ethidium bromide will work. At
the low levels used neither interferes with the PCR. The Lightcycler
Reference given for the Biotechniques paper is the one to look at.
I'll tell you how well it works on a Lightcycler on Monday.
The problem with being on the cutting edge is that you occasionally get
sliced from time to time....
More information about the Methods