Frog skin DNA isolation

[Nick Theodorakis]

In <20010821114835.26689.qmail at ww02.jatek.com>, Darnel wrote:
>
>I need skin DNA because specialized cells locally found in the skin 
produce
>an antimicrobial-peptide there. Therefore that gene coding for that 
peptide
>is expressed there.Although every cell of the body contains the same 
DNA,
>specialised cells will switch certain genes on and off. It is more likely
>that I will find the gene there than in say, a muscle or a liver
>

I'm not following you here. Does this gene undergo tissue-specific 
rearrangement as the immunoglobulin genes do? If not, then any DNA 
will suffice, even from blood, which you can probably get easily without 
killing the frog, if you wish (and since erythrocytes are nucleated in 
amphibians, there should be plenty of DNA in a little blood).

Maybe you should give us some more info. Are you making cDNA first?

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu