Glycerol stock longevity
jakku at mrna.tn.nic.in
Thu Jun 7 23:29:01 EST 2001
we too do it exactly the same way.. and it lasts for years and years....
Dept. of Molecular Microbiology,
School of Biotechnology,
Madurai Kamaraj University,
Madurai - 625021. India.
Email: jakkku at mrna.tn.nic.in
Science is constructed on facts like a house is of bricks, but a mere
accumulation of facts does not make science nor a pile of bricks a house
----- Original Message -----
From: "Michael Witty" <mw132 at mole.bio.cam.ac.uk>
To: <methods at hgmp.mrc.ac.uk>
Sent: Friday, June 08, 2001 12:57 AM
Subject: Re: Glycerol stock longevity
> My glycerols are 0.5ml overnight culture plus 0.5ml of 80%w/v glycerol
> that I freeze on dry ice (when I can) or just put into the minus seventy.
> I am very careful to keep them cold, so when I sub I take them out and
> scrape some stuff of the top and put them back VERY FAST or better yet,
> keep them on dry ice. Make duplicates in case of accidents though. They
> last for years and years. This works for E coli and Agrobacterium in
> my experience. Any other experience out there? Mike.
> On 7 Jun 2001, Betsy Moran wrote:
> > Hi,
> > Does any one have information (data or opinion or personal experience)
> > how
> > long a bacterial glycerol stock is viable @ -80oC? How often to stocks
> > to
> > regrow? Is is necessary to thaw and revitalize the stock at time
> > Just curious...
> > Betsy
> > bam_work at hotmail.com
> > _________________________________________________________________
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