Dead stab culture - what to do?
tk at suspiria.ai.mit.edu
Wed Mar 6 13:48:05 EST 2002
Cornelius Krasel <krasel at wpxx02.toxi.uni-wuerzburg.de> writes:
> I seem to have a dead stab culture (at least I didn't manage to get
> any colonies from it by taking out some agar and spreading it on a
> plate) from which I would like to recover the plasmid somehow.
As a last resort, you might try colony (stab?) PCR using some primers
which can be recircularized to give you the vector.
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