Dead stab culture - what to do?

[Tom Knight]

Cornelius Krasel <krasel at wpxx02.toxi.uni-wuerzburg.de> writes:
> I seem to have a dead stab culture (at least I didn't manage to get
> any colonies from it by taking out some agar and spreading it on a
> plate) from which I would like to recover the plasmid somehow.

As a last resort, you might try colony (stab?) PCR using some primers
which can be recircularized to give you the vector.