E. coli Question
Gary
gp95ac at brocku.ca
Wed Feb 18 11:26:00 EST 2004
Hey folks,
Does anyone have an approximation of how much dry cell weight would be
contained in a wet cell pellet of E. coli??
I know this should be determined empirically, but surely someone who
works with E. coli regularly should have an idea.
Would it be 50% of the weight = dry cell mass (or another percentage)?
So some detail
E.coli K12 (37C, 220rpm) overnight, saturated culture
Spin down, discard media, aspirate off any remaining.
Thanks for the tips!!
Gary
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