E. coli Question

Gary gp95ac at brocku.ca
Wed Feb 18 11:26:00 EST 2004


Hey folks,

Does anyone have an approximation of how much dry cell weight would be
contained in a wet cell pellet of E. coli??

I know this should be determined empirically, but surely someone who
works with E. coli regularly should have an idea.

Would it be 50% of the weight = dry cell mass (or another percentage)?

So some detail

E.coli K12 (37C, 220rpm) overnight, saturated culture

Spin down, discard media, aspirate off any remaining.

Thanks for the tips!!

Gary



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