Ligation problems

[Tim Fitzwater]

"Why do I have so many white colonies that result in a plasmid with no
insert?" (Stratagene FAQ at their website)
"It is imperative that you use at least a 40:1 insert:vector ratio in the
ligation. Too low an insert concentration will result in false positives due
to the increased enzymatic activity and constant cutting and religating of
the vector ends. Bases can be lost in this situation causing a frameshift
which results in a white colony."

Tim Fitzwater
SomaLogic
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