[Methods-and-reagents] Re: Expressing high activity AP in E. coli

[Duncan Clark]

Historians believe that in newspost 
<mailman.39.1120430358.29381.methods at net.bio.net> on Sun, 3 Jul 2005, 
"Yoram Gerchman (gerchman at Princeton.EDU)" <gerchman at Princeton.EDU> 
penned the following literary masterpiece:
>I want to express an high activity alkaline phosphatase in E. coli. Anyone has an idea for high activity AP gene that work in E. coli? I know Roche
>got a recombinant version of the bovine AP but I could not find any reference as to how it was done.

If you have done the pre-requisite Google and Pubmed searches then next 
stop is to have to look at patents i.e. US5434067

The European patent office have a good search engine and you can view 
the full patent/application for EU, US and Worldwide.

http://ep.espacenet.com/search97cgi/s97_cgi.exe?Action=FormGen&Template=ep/en/home.hts

Duncan
-- 
I love deadlines. I especially like the whooshing noise they make as
they go flying by.

Duncan Clark
GeneSys Ltd.