pAS1 and pAS1-CYH2 vector

Jianxiang Liu jianxliu at iastate.edu
Tue Mar 1 20:47:40 EST 2005


Hi there
I got the library from ABRC for Y2H. In the kit there are four Ecoli strains. 
Does anybody know which antibiotics I should add if I want to growth those 
bacterial strains (pAS1, pAS1-CYH2, strain for library amplification, strain 
for library excision) in LB medium to make the frozen stock? Does anybody has 
the pAS1 map in details? I could not find the selecting markers in the vector 
map. Thank you so much in advance.
Regards
Jianxiang

Jianxiang Liu
1073 Roy J. Carver Co. Lab
Department of Genetics, Development and Cell Biology
Iowa State University
Ames, Iowa, 50011-3650
Lab Tel: 515-294-4966



---



More information about the Methods mailing list