ideal medium for HepG2 cells?

[Nick Theodorakis]

On Sat, 05 Mar 2005 01:28:11 GMT, dk at no.email.thankstospam.net (D.K.)
wrote:

>In article <6.2.1.2.0.20050304204900.0275c810 at pop.gmx.net>, hubahopp at gmx.de (Wolfgang Schechinger) wrote:
>>Dear all,
>>
>>some people grow HepG2 cells with lots of additions like Aprotinin, 
>>Insulin, Transferrin, alpha Fetoprotein etc..
>>
>>The ATCC manual just suggests RPMI with 10% serum.
>>
>>When does it make sense to add all that expensive stuff?
>
>When it's serum free medium. Serum already contains all that stuff
>(or better).  Don't know about RPMI though. My memory tells me 
>that all HepG2 culture I saw was in DMEM/F-12. 
>

I used to grow them in MEM-alpha with 10% FCS.

Nick


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