ideal medium for HepG2 cells?
novalidaddress at nurfuerspam.de
Mon Mar 7 15:22:37 EST 2005
the story now seems to be another example of evolutionary development of
Long long time ago, one somebody grew them in serum free medium for some
reason, added the named stuff, the next guy used ordinary medium but was
afraid his cells would die if he wouldn't give them the goodies, the next
guy introduced some errors while copywriting and so on. Just the concept of
creating variation in a non-genomic way :-)
ENOUGH MUTATIONS NOW! SELECTION WILL START!
After 3 days in some leftover DMEM w/ glutamax and 10% (from last
december), they look happier and healthier than ever!
Best regards, Wolfgang
At 20:05 07.03.2005, you wrote:
>In all the years I've grown HepG2, I've never grown them in anything but
>DMEM High glucose/ high-bicarb, usual amounts of L-glut, 10% FBS,
>Penn/strep, and I've often thrown in Sodium pyruvate when available (though
>(When being very lazy, I had an incubator full of cells requiring DMEM and
>one that required RPMI1640. I still used the above media but tossed in the
>required amount of non-essential amino acids.)
>So, no. You don't need all those expensive goodies unless there is some
>compelling reason the cells need to be a certain way before any experiments.
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