customer at newsnet.com
Sat Mar 25 00:33:24 EST 2006
> > For electroporation yes because many years ago (I have been around
> > that long!) it was reported that the T4 DNA ligase really lowers the
> > electroporation efficiency. Heat killing that was the key to fixing
> > the problem.
> While this is probably true for normal ligation buffers, NEB
> recommends against heat killing ligase in the quick ligation buffers.
> Our experiments confirm about a 100x reduction in
> ligation/transformation efficiency due to heat killing ligase in this
I won't be doing transformation via electroporation.
I will be using heat shock competant E.coli.
Would it be fair to say that I wont need to heat to kill ligase in my
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