purification of radioactive probe
(by marker from rhrk.uni-kl.de)
Mon Aug 27 08:06:14 EST 2007
does anyone know if it is really necessary to purify a radioactive DNA probe
after labelling it by random priming? I want to use it on a northern blot.
So far, I worked with DIG-labelled probes, that do not need to be purified
after labelling. They are used directly in the hybridisation solution. Is it
different with radioactive probes? Do you think that precipitation by salt
and alcohol is sufficient for purification?
Thank you very much,
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