DNA binding protein purification

[Wenta Nikola]

Hello Itisam,
our lab also works with DNA binding proteins. We isolate that protein from Sf9 cells and also from bacteria cells. After lysis, we don't do DNase digest but instead use 0.1% (v/v) PEE (Polyethylenimine) from Sigma (P-3143) to get rid of DNA. For our protein, it works well; maybe you need to check your protein's pI because PEE might also precipitate your protein ;-).
Cheers,
Niko

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