protocol for liposome preparation

[jh]

Excuse me, may you explain more detail for "PC".
Whether the liposome is prepared for cell transfection experiment?

Jianhao

2008/11/30 WS <novalidaddress from nurfuerspam.de>:
> Hi Michael,
>
> you might try this: dissolve PC in EtOH and inject into 20 to 50 fold
> volume of buffer with an insulin syringe (or the narrowest needle you
> can get) while vortexing. If the EtOH might cause problems downstream,
> dialyze against buffer.
>
> Worked well for PC/Ceramide vesicles for me.
>
> best regards,
>
> Wo
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>