protocol for liposome preparation

[WS]

On 3 Dez., 15:18, jh <bio... from gmail.com> wrote:
> Excuse me, may you explain more detail for "PC".
> Whether the liposome is prepared for cell transfection experiment?
>
> Jianhao
>
> 2008/11/30 WS <novalidaddr... from nurfuerspam.de>:
>
> > Hi Michael,
Hi,

PC means phosphatidylcholine. I haven't used them for transfection but
to transfer a fluorescently labeled ceramid into fibroblasts.

Regards,

Wo


> > you might try this: dissolve PC in EtOH and inject into 20 to 50 fold
> > volume of buffer with an insulin syringe (or the narrowest needle you
> > can get) while vortexing. If the EtOH might cause problems downstream,
> > dialyze against buffer.
>
> > Worked well for PC/Ceramide vesicles for me.
>
> > best regards,
>
> > Wo
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