Size-Exclusion

[Jan R. Krähling]

Hello!

 

We are trying to establish the sixe-exclusion chromatography in our lab to purify the enzyme of our interest. In the purification-steps before the  size exclusion we observe that a NaCl-concentration of 1M stabilize the enzyme better than lower NaCl concentrations. So we tried the size exclusion at first with a high salt concentration, but the enzyme wasn't detectable. At lower concentrations the enzyme is detectable and active. My question is, if it is possible, that through the size-exclusion the salt-concentration increased to such a high level, that this will damage the enzyme. Is it possible that the elution of NaCl is so strong delayed, that at the top of the column unexpected high concentration of NaCl occur?

 

Thanks for your help!

 

 

Jan









Jan R. Krähling
Pharmacist
Institute of Pharmacology, Toxicology and Clinical Pharmacy
Technical University of Brunswick
Mendelssohnstrasse 1
D-38106 Braunschweig
E-Mail:    J-R.Kraehling from tu-braunschweig.de
Tel.         05 31 / 391 - 56  23
Fax.        05 31 / 391 - 81 82