Gewtting the concentration of PCR product and cloning

[yoginee budhkar]

Hello Shifali,

Your first question is answered correctly already, for the quatification,
read on...

OD of 1 at 260nm corresponds to 50 μg/ml for double-stranded DNA.
Simply take absorbance of your PCR product on a nanophotometer/ a nanodrop.

Or dilute it suitably to 1 ml and take A260 in a quartz cuvette, in any
spectrophotometer.
Multiply it with the dilution factor to find out the concentration of you
PCR product.

--Yg



> On Thu, 25 Dec 2008 Shri wrote :
> >Hi all members,
> >I have amplified PCR product of 1250 bp from Genomic DNA
> >  my question is
> >1 Whether it can be cloned in TA cloning vector the required size.
> >2. how to calculate the concentration of the PCR product after the
> >reaction
> >_______________________________________________
> >Methods mailing list
> >Methods from net.bio.net
> >http://www.bio.net/biomail/listinfo/methods
>
>
> Shifali Chatrath
> Graduate Student
> Protein science Lab
> Dept. of Biological sciences
> National University of Singapore
> Singapore
> +65-65161210
> +65-96393449
>
> ------------------------------
>
>