Media left at 37 C O/N?

[Tim Eggleston]

The question regarding heating the RPMI media to 60 C brought to mind
a question I've wondered about from time to time.  If media, such as
DMEM or MEM, is left in a 37 C water bath O/N, will be ok to use it
the next day.  I have always advised people to pitch it due to the
possible degradation of L-glutamine, but I have always wondered how
much degradation actually occurs, if any.  Thanks!

-- 
Tim Eggleston
Horne Lab
Research Assistant
2-505 Bowen Science Building
University of Iowa