pcr prob

[Aawara Chowdhury]

In <1203946852.17846.0 from proxy01.news.clara.net>,
 ChenHA <hzhen from freeuk.com> wrote:

> Aawara Chowdhury wrote:
>
>> P.S. You don't have to be so rude, just because someone points out
>> an incongruence in your "observation".
>> 
>
> Geez, now you are truly stupid.  Why put the quotation marks on 
> observation?  Are you suggesting that observations were wrong, or false, 
> or I have mistaken a reflection of a light bulb to be a band?  What's 
> incongruent about my observation?  Observations are observations, 
> science comes out of observation, idiot.  I have shown that you made 
> unwarranted assumption on primer concentration and length, and you still 
> insist on piling on casting doubt on my "observation".  Stupid people 
> deserve all the insult they get.

Your "observation" is incongruent with everything that has been published
about the function of ethidium bromide.  You "observe" that ethidium
fluoresces "brightly" when bound to single-stranded oligonucleotides.
That either means that your oligonucleotides were not single-stranded
(odd for PCR primers), or that there's an error with your "observation".
And yes, that is precisely why I find your "observation" perplexing.
And unfortunately for you, science is not just about making an "observation";
it also requires being prepared to subject an observation to review.  

And my, don't we like calling people stupid.  Makes you feel intelligent,
does it?

AC
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