pcr prob

Aawara Chowdhury via methods%40net.bio.net (by aawara from pontiff-playground.org)
Wed Feb 27 22:02:15 EST 2008


In <a6f52391-02f5-456e-9800-7027c4dab765 from d5g2000hsc.googlegroups.com>,
 Tom Landers <galaxyglue from gmail.com> wrote:

> Supposedly the Sybr dyes interact with the sugar-phosphate backbone,
> unlike ethidum's base intercalating (thus its specificity for dsDNA).

The original - SybrGreen was very specific for dsDNA.  SybrGold definitely
binds dsDNA, ssDNA, and RNA.  

SybrSafe is guaranteed to bind dsDNA, but it doesn't say that it doesn't
bind the others. 

AC
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