Albumin depletion from sheep plasma

[Pow Joshi]

On 03/03/2008, Arianmanesh, Mitra <r06ma6 from abdn.ac.uk> wrote:
>
> Dears
>
> I need to deplete Albumin from sheep plasma while there is no defined
> and perfect method for it. Actually, I used several methods for that
> such as Immunoprecipitation and SwellGel Blue Albumin Removal Kit but I
> could not get good results. So, now I need to know that how to deplete
> Abumin from the sheep plasma. Can anybody give me an advise to do that



yes, that is a tough one.. Perhaps, you could just use Blue sepharose  CL-6B
(trade name I think is Cibacron), and run your plasma one it as a column a
few times or perhaps do a serial culk incubation.
Now, if you wish to purify antibodies, you could use the afffinity columns;
if you don't care about having or not having antibodies in the plasma/serum,
you could probably use an ion exchanger as well. ...actually, I think I am a
bit fuzzy here, and can be able to help you better if you let us know what
exactly you wish to get out of the plasma.

best
Pow

well?
>
> Kind regards,
>
> Mitra
>
>
>
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