solid phase colony growth

Clive Tregaskes via methods%40net.bio.net (by meltonbf2007 from tragoc.f2s.com)
Tue Mar 4 18:15:43 EST 2008


Hi all,
some time ago there was a paper (I think it was a NAR methods paper)
regarding growing bacteria for cDNA libraries etc. in a semi-solid gel
rather than on plates so (as the authors claimed) the colonies all grew to
the same size and you didn't lose representation.
Anyone have any idea where this paper came from- I've tried various searches
and I can't find it again- otherwise anybody got the method- Its something
simple like suspending the cells in LB/1% low melting point agar but not
certain and can't remember how you get the bacteria back out again
all help gratefully appreciated
Clive



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