Maurizio Affer <borderline86 from yahoo.com> wrote: >I induce at an OD of 0.8 with IPTG final of 0.1mM for >2 hours. This is a quite short time after induction and also a very small concentration for the IPTG. We ususally induce for 3-5 hours with 1mM IPTG final using pGEX and BL21. Christian -- X-no-Sig: yes