"freeze and squeeze" DNA extraction

[Sudheendra Rao N R]

I use this method.QIAEX II particle.
http://www1.qiagen.com/literature/handbooks/literature.aspx?id=1000202

try.
sudhee.

On Sun, Mar 16, 2008 at 3:19 PM, Dunowska, Magda <M.Dunowska from massey.ac.nz>
wrote:

> Hi,
>
> Sorry to revisit the subject - I have just gone through the archives and
> can't find a description of a method I used to use a few years ago to get
> DNA from agarose gels. I used normal (not LMP) agarose and TBE buffer. All I
> remember is that it involved freezing and thawing a gel slice, but I think I
> was also adding water or TE (can't remember what and how much), heating the
> thawed gel to either 65 or 70 degrees for 5 or 10 minutes (?) and mashing it
> all up with the blue tip before centrifuging everything at 4 degrees to
> collect supernatant.
>
>
>
> I just got back to the molecular work after a few years in a slightly
> different role, and (surprisingly...) can't recall all the details of
> procedures that I used to do on a daily basis and never bothered to write
> down outside of the lab books that are no longer with me...Anybody out there
> who uses/used a similar variant of the "freeze and squeeze" and can give me
> the details?
>
> Thanks!
>
> Magda
>
>
>
>
> _______________________________________________
> Methods mailing list
> Methods from net.bio.net
> http://www.bio.net/biomail/listinfo/methods
>



-- 
Think before agree
Think before you nod
but STOP thinking
and You Are God