"freeze and squeeze" DNA extraction

[Farahani Poupak]

I just use "Freeze 'N Squeeze DNA Gel Extraction Kit"
from Bio-Rad. I don't have any variants for this
protocol. 




--- Sudheendra Rao N R <sudhee26 from gmail.com> wrote:

> I use this method.QIAEX II particle.
>
http://www1.qiagen.com/literature/handbooks/literature.aspx?id=1000202
> 
> try.
> sudhee.
> 
> On Sun, Mar 16, 2008 at 3:19 PM, Dunowska, Magda
> <M.Dunowska from massey.ac.nz>
> wrote:
> 
> > Hi,
> >
> > Sorry to revisit the subject - I have just gone
> through the archives and
> > can't find a description of a method I used to use
> a few years ago to get
> > DNA from agarose gels. I used normal (not LMP)
> agarose and TBE buffer. All I
> > remember is that it involved freezing and thawing
> a gel slice, but I think I
> > was also adding water or TE (can't remember what
> and how much), heating the
> > thawed gel to either 65 or 70 degrees for 5 or 10
> minutes (?) and mashing it
> > all up with the blue tip before centrifuging
> everything at 4 degrees to
> > collect supernatant.
> >
> >
> >
> > I just got back to the molecular work after a few
> years in a slightly
> > different role, and (surprisingly...) can't recall
> all the details of
> > procedures that I used to do on a daily basis and
> never bothered to write
> > down outside of the lab books that are no longer
> with me...Anybody out there
> > who uses/used a similar variant of the "freeze and
> squeeze" and can give me
> > the details?
> >
> > Thanks!
> >
> > Magda
> >
> >
> >
> >
> > _______________________________________________
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> > Methods from net.bio.net
> > http://www.bio.net/biomail/listinfo/methods
> >
> 
> 
> 
> -- 
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> Think before you nod
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